Human Lipoprotein A Matched Antibody Pair Kit (ab217612)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 15.625 ng/ml - 1000 ng/ml
Overview
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Product name
Human Lipoprotein A Matched Antibody Pair Kit
See all Lipoprotein a kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1890 pg/ml -
Range
15.625 ng/ml - 1000 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Lipoprotein A Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Lipoprotein A.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab212165. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Lipoprotein A Capture Antibody 2 x 50µg 1 x 50µg Human Lipoprotein A Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Lipoprotein A Lyophilized Protein 2 vials 1 vial -
Research areas
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Relevance
Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis. -
Alternative names
- AK38
- Antiangiogenic AK38 protein
- apo(a)
see all -
Database links
- Entrez Gene: 4018 Human
- Omim: 152200 Human
- SwissProt: P08519 Human
- Unigene: 520120 Human
Images
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Human Lipoprotein A standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Lipoprotein A Matched Antibody Pair Kit (ab217612)To learn more about the advantages of recombinant antibodies see here.
