Human Lipoprotein A ELISA Kit (ab108878)
Key features and details
- Sensitivity: 0.7 ng/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Lipoprotein A ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.6% Inter-assay Sample n Mean SD CV% Overall 9.5% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.7 ng/ml -
Range
0.78 ng/ml - 50 ng/ml -
Recovery
= 98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Lipoprotein A Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lipoprotein A concentrations in plasma, serum, urine, milk, cerebrospinal fluid (CSF) and cell culture supernatants.
A Lipoprotein A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lipoprotein A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lipoprotein A captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Lipoprotein A Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Lipoprotein A Microplate (12 x 8 well strips) 1 unit Lipoprotein A Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330. -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 38 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsN- and O-glycosylated. The N-glycans are complex biantennary structures present in either a mono- or disialylated state. The O-glycans are mostly (80%) represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated and non-sialylated O-glycans also detected. - Information by UniProt
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Alternative names
- Apo(a)
- APOA_HUMAN
- Apolipoprotein(a)
see all -
Database links
- Entrez Gene: 4018 Human
- SwissProt: P08519 Human
- Unigene: 520120 Human
Images
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Sandwich ELISA - Lipoprotein A Human ELISA Kit (ab108878)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Sandwich ELISA - Lipoprotein A Human ELISA Kit (ab108878)Lipoprotein A measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum, plasma and milk samples diluted 4000-32000 fold. Human urine and saliva diluted 2-8 fold. Mouse and rat samples diluted 10-100 fold. Cell supernatants diluted 1-10 fold.
