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Cardiovascular Lipids / Lipoproteins Lipoproteins/Apolipoproteins Lipoproteins

Anti-Lipoprotein a antibody [EPR6474] (ab125014)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Lipoprotein a antibody [EPR6474] (ab125014)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6474] to Lipoprotein a
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Lipoprotein a antibody [EPR6474]
    See all Lipoprotein a primary antibodies
  • Description

    Rabbit monoclonal [EPR6474] to Lipoprotein a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lipoprotein a aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Human plasma lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6474
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Lipoproteins
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Apolipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)
    Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)
    Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution (purified) + Human plasma lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

    Predicted band size: 501 kDa
    Observed band size: 501 kDa



    Blocking and dilution buffer: 5% NFDM /TBST.

    The observed bands are consistant with desciption in PMID:10703674, which should be N-terminal fragments.

  • Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)
    Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)
    Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution (unpurified) + Human plasma lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 501 kDa
    Observed band size: 500 kDa
    why is the actual band size different from the predicted?

  • Anti-Lipoprotein a antibody [EPR6474] (ab125014)
    Anti-Lipoprotein a antibody [EPR6474] (ab125014)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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