Anti-Lipoprotein a antibody [EPR6474] (ab125014)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6474] to Lipoprotein a
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Lipoprotein a antibody [EPR6474]
See all Lipoprotein a primary antibodies -
Description
Rabbit monoclonal [EPR6474] to Lipoprotein a -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Lipoprotein a aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: Human plasma lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6474 -
Isotype
IgG -
Research areas
Images
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Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution (purified) + Human plasma lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 501 kDa
Observed band size: 501 kDaBlocking and dilution buffer: 5% NFDM /TBST.
The observed bands are consistant with desciption in PMID:10703674, which should be N-terminal fragments.
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Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution (unpurified) + Human plasma lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 501 kDa
Observed band size: 500 kDa why is the actual band size different from the predicted?
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