Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15404(B)] to IMPA1
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IMPA1 antibody [EPR15404(B)]
    See all IMPA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15404(B)] to IMPA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HCT116 and PC3 cells; Jurkat, PC3, HCT116 aand Human testis lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Western blot - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    All lanes : Anti-IMPA1 antibody [EPR15404(B)] (ab184165) at 1/50000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : PC 3 cell line lysate
    Lane 4 : HCT116 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

    Immunofluorescent analysis of HCT116 cells (4% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

    Western blot analysis of immunoprecipitation pellet from Human testis tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184165 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Flow Cytometry - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Flow Cytometry - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

    Flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-IMPA1 antibody [EPR15404(B)] (ab184165)
    Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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