Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15404(B)] to IMPA1
Suitable for: Flow Cyt, WB, IP, ICC/IF
Reacts with: Human

Product name

Anti-IMPA1 antibody [EPR15404(B)]
See all IMPA1 primary antibodies
Description

Rabbit monoclonal [EPR15404(B)] to IMPA1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HCT116 and PC3 cells; Jurkat, PC3, HCT116 aand Human testis lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15404(B)
Isotype

IgG
Research areas

Western blot - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

All lanes : Anti-IMPA1 antibody [EPR15404(B)] (ab184165) at 1/50000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : PC 3 cell line lysate
Lane 4 : HCT116 cell line lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Immunofluorescent analysis of HCT116 cells (4% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Western blot analysis of immunoprecipitation pellet from Human testis tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184165 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Flow Cytometry - Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-IMPA1 antibody [EPR15404(B)] (ab184165)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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