Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free
    See all alpha Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, A431 and K562 cell lysates; Human kidney and uterus tissues; A431 and Jurkat cells.

  • General notes

    Ab220805 is the carrier-free version of ab176560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220805 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Immunofluorescent staining of A431 cells fixed in 4% PFA with purified ab176560 at a dilution of 1/350. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Immunofluorescent staining of A431 cells fixed in 4% PFA with unpurified ab176560 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

  • Flow Cytometry - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Flow Cytometry - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Flow cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purified ab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Flow Cytometry - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

    Flow cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176560).

  • Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)
    Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free (ab220805)

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