Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634)
Key features and details
- Rabbit polyclonal to Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody -
Description
Rabbit polyclonal to Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) -
Host species
Rabbit -
Specificity
ab193634 detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318 respectivly.
-
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human Ephrin B1 aa 300-400 (phospho Y324) conjugated to keyhole limpet haemocyanin. Swiss-Prot: P98172/P52799/Q15768.
-
Positive control
- Extracts from HepG2 cells treated with serum. Human brain tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab193634 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634)All lanes : Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells treated with serum
Lane 2 : Extracts from HepG2 cells treated with serum with antigen-specific peptide
Predicted band size: 47 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634)Immunohistochemical analysis of paraffin-embedded Human brain tissue sections labeling Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) with ab193634 at a 1/50 dilution. Right image is treated with blocking peptide.
