Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1047] to ErbB2 / HER2 (phospho Y1221 + Y1222) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free
    See all ErbB2 / HER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR1047] to ErbB2 / HER2 (phospho Y1221 + Y1222) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects ErbB2 / HER2 phosphorylated at tyrosine 1222.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247528 is the carrier-free version of ab91633 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
    Western blot - Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
    All lanes : Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] (ab91633) at 1/5000 dilution

    Lane 1 : SKBR3 cell lysate was untreated
    Lane 2 : SKBR3 cell lysate was treated with Lambda phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 138 kDa



    This data was developed using ab91633, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
    Dot Blot - Anti-ErbB 2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
    This data was developed using ab91633, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/1000 Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated Secondary antibody dilution: 1/2500 Blocking & dilution buffer: 5% NFDM/TBST Lane 1 sample: ErB 2 (pY1221/pY1222) phospho peptide Lane 2 sample: ErB 2 non-phospho peptide Exposure time: 3 minutes
  • Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)
    Anti-ErbB2 / HER2 (phospho Y1221 + Y1222) antibody [EPR1047] - BSA and Azide free (ab247528)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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