Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19140] to TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free
    See all TrkA+TrkB+TrkC primary antibodies

  • Description

    Rabbit monoclonal [EPR19140] to TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251238 is the carrier-free version of ab197071 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251238 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    R

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution

    Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab197071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/10000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (phospho Y516A mutant) with Myc-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab197071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers (PMID: 23115189 and 20064930).

  • Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    Western blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours, then treated with 50 ng/ml BDNF for 10 minutes
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB (wild type) with Myc-tag starved for 4 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 92 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab197071, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is higher than the predicted one due to the glycosylation. The expression pattern is consistent with the published papers (PMID: 23115189 and 20064930).

  • Dot Blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    Dot Blot - Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    This data was developed using ab197071, the same antibody clone in a different buffer formulation.Dot blot analysis of TrkA (phospho Y496), TrkB (phospho Y516), and TrkC (phospho Y516) peptides labeled with ab197071 at 1/1000 dilution. Lane 1: TrkB (phospho Y516) phospho peptide; Lane 2: TrkB Non-phospho peptide; Lane 3: TrkA (phospho Y496) phospho peptide; Lane 4: TrkA Non-phospho peptide; Lane 5: TrkC (phospho Y516) phospho peptide; Lane6: TrkC Non-phospho peptide. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes. Note: ab197071 reacts with TrkB (phospho Y516)/ TrkA (phospho Y496)/ TrkC (phospho Y516) according to the dot blot data.
  • Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)
    Anti-TrkA+TrkB+TrkC (phospho Y496 + Y516 + Y516) antibody [EPR19140] - BSA and Azide free (ab251238)

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