Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
![Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)](https://www.abcam.com/ps/products/247/ab247365/Images/ab247365-4-anti-ngf-antibody-ep1318y-bsa-and-azide-free-flow-cytometry.jpg "Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1318Y] to NGF - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NGF antibody [EP1318Y] - BSA and Azide free
See all NGF primary antibodies -
Description
Rabbit monoclonal [EP1318Y] to NGF - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognizes pro-NGF.
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Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247365 is the carrier-free version of ab68151. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247365 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1318Y -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)This data was developed using ab68151, the same antibody clone in a different buffer formulation.Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
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![Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)](https://www.abcam.com/ps/products/247/ab247365/Images/ab247365-405504-anti-ngf-antibody-ep1318y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365) -
![Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)](https://www.abcam.com/ps/products/247/ab247365/Images/ab247365-402297-anti-ngf-antibody-ep1318y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365) -
![Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)](https://www.abcam.com/ps/products/247/ab247365/Images/ab247365-399259-anti-ngf-antibody-ep1318y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)
Lane 1 : 10ng human pro-NGF recombinant protein
Lane 2 : 5ng human pro-NGF recombinant protein
Lane 3 : 1ng human pro-NGF recombinant protein
Lane 4 : 0.5ng human pro-NGF recombinant protein
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 130 kDa, 20 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab68151, the same antibody clone in a different buffer formulation.
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![Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)](https://www.abcam.com/ps/products/247/ab247365/Images/ab247365-5-benefits-of-recombinant-antibodies.png)
Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
