Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1318Y] to NGF - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NGF antibody [EP1318Y] - BSA and Azide free
    See all NGF primary antibodies

  • Description

    Rabbit monoclonal [EP1318Y] to NGF - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes pro-NGF.

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247365 is the carrier-free version of ab68151. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247365 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Flow Cytometry - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    This data was developed using ab68151, the same antibody clone in a different buffer formulation.Overlay histogram showing SH-SY5Y cells stained with ab68151 (red line) at 1/280 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
  • Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Rat brain tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab68151, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Anti-NGF antibody [EP1318Y] (ab68151) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

    Predicted band size: 27 kDa



    This data was developed using ab68151, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Western blot - Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    All lanes : Anti-NGF antibody [EP1318Y] (ab68151) at 1/5000 dilution (unpurified)

    Lane 1 : 10ng human pro-NGF recombinant protein
    Lane 2 : 5ng human pro-NGF recombinant protein
    Lane 3 : 1ng human pro-NGF recombinant protein
    Lane 4 : 0.5ng human pro-NGF recombinant protein

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa
    Additional bands at: 130 kDa, 20 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab68151, the same antibody clone in a different buffer formulation.

     

  • Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)
    Anti-NGF antibody [EP1318Y] - BSA and Azide free (ab247365)

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