Antibodies, Proteins, Kits and Reagents for Life Science

Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (ab192055)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Alexa Fluor® 488 Rabbit monoclonal [EP1612Y] to Cytokeratin 14
Suitable for: Flow Cyt, ICC
Knockout validated
Reacts with: Human
Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Product name

Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y]
See all Cytokeratin 14 primary antibodies
Description

Alexa Fluor® 488 Rabbit monoclonal [EP1612Y] to Cytokeratin 14
Host species

Rabbit
Conjugation

Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applications

Suitable for: Flow Cyt, ICCmore details
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC: A431 cells. Flow Cyt: A431 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1612Y
Isotype

IgG
Research areas

Immunocytochemistry - Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (ab192055)

ab192055 staining KRT14 in wild-type A431 cells (top panel) and KRT14 knockout A431 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192055 at 1/100 dilution and ab190573 (Rabbit monoclonal to alpha Tubulin - Alexa Fluor^® 647) at 1/250 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (ab192055)

Overlay histogram showing A431 cells stained with ab192055 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192055, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in A431 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (ab192055)

ab192055 staining Cytokeratin 14 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192055 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor^® 594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
This product also gave a positive signal in 4% formaldehyde (10 min) fixed A431 cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).
Flow Cytometry - Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (ab192055) This image is courtesy of an anonymous Abreview

ab192055 staining Cytokeratin 14 in CK14 bright positive and low cell lines by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with PBS + 5% FBS + 2% Triton X-100. The sample was incubated with the primary antibody (1/50 in PBS + 5% FBS + 0.2% Triton X-100) for 45 minutes at 4°C.
Gating Strategy: Doublet were excluded based on SSC - FSC profile.
See Abreview
Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (ab192055)

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