Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free
    See all Cytokeratin 3/CK-3 primary antibodies

  • Description

    Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow Cyt: A431 cells. IHC-P: Human squamous papilloma of cornea tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255962 is the carrier-free version of ab68260. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab68260).

    Immunohistochemical analysis of paraffin-embedded human squamous papilloma of cornea tissue labeling Cytokeratin 3/CK-3 with ab68260 at 1/500 dilution, followed by ready to use secondary. Positive staining on human squamous papilloma of cornea tissue is observed. The section was incubated with ab68260 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Flow Cytometry - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)
    Flow Cytometry - Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab68260).

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cell line labeling Cytokeratin 3/CK-3 with ab68260 at 1/500 dilution (red) compared with a Mouse IgG, monoclonal Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG ab150113 (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)
    Anti-Cytokeratin 3/CK-3 antibody [AE5] - BSA and Azide free (ab255962)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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