Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16813] to Apolipoprotein CI/Apo-CI
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813]
    See all Apolipoprotein CI/Apo-CI primary antibodies

  • Description

    Rabbit monoclonal [EPR16813] to Apolipoprotein CI/Apo-CI

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and Human plasma lysate. IHC-P: Human liver tissue. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells. IP: Human plasma whole cell extract .

  • General notes

     This product was previously labelled as Apolipoprotein CI

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/1000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 9 kDa
    Observed band size: 6.6 kDa
    why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Western blot - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288) at 1/5000 dilution + Human plasma lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 9 kDa
    Observed band size: 6.6 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/800 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198288 at 1/800 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Flow Cytometry - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Apolipoprotein CI/Apo-CI with ab198288 at 1/80 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) (ab172730) and cell without incubation with primary antibody and secondary antibody is blue.

  • Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Immunoprecipitation - Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

    Immunoprecipitation analysis of Human plasma whole cell extract labeling Apolipoprotein CI/Apo-CI using ab198288 at 1/40 dilution (Lane 2).
    Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198288 in Human plasma whole cell extract.
    Lane 1: Input: 10 μg Human plasma whole cell extract.
    Subsequent WB detection was performed using ab198288 at 1/1000 dilution.
    An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)
    Anti-Apolipoprotein CI/Apo-CI antibody [EPR16813] (ab198288)

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