Anti-Angiopoietin 2/ANG2 antibody (ab8452)
Key features and details
- Rabbit polyclonal to Angiopoietin 2/ANG2
- Suitable for: ICC/IF, WB
- Reacts with: Rat, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Rabbit polyclonal to Angiopoietin 2/ANG2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, African green monkey -
Immunogen
Synthetic peptide corresponding to Mouse Angiopoietin 2/ANG2 aa 21-40 (N terminal).
Sequence:CNNFRKSVDSTGRRQYQVQNGP
(Peptide available asab9077) -
General notes
This product was previously labelled as Angiopoietin 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Angiopoietin 2/ANG2 antibody (ab8452) This image is courtesy of Marion Scharpfenecker 2002
Supernatants of mouse angiopoietin-expressing endothelial cells. Soluble Tie 2 was used to precipitate the angiopoietins to reduce background.
Lane 1 - mock
Lane 2 - mouse angiopoietin-2 (clone 2-9) expressing cells
Lane 3 - mouse angiopoietin-1 (clone 1-15) expressing cells
Lane 4 - mouse angiopoietin-1 (clone 1-8) expressing cells
Lane 5 - wt -
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (ab8452)ICC/IF image of ab8452 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8452, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
