Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6253] to PSMA
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-PSMA antibody [EPR6253]
See all PSMA primary antibodies
Description

Rabbit monoclonal [EPR6253] to PSMA
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human PSMA aa 1-150 (N terminal). The exact sequence is proprietary.
Database link: Q04609
Positive control
- Human prostate carcinoma tissue; LNCaP cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6253
Isotype

IgG
Research areas

Western blot - Anti-PSMA antibody [EPR6253] (ab133579)

Anti-PSMA antibody [EPR6253] (ab133579) at 1/100000 dilution (Purified) + LNCaP lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L)

Predicted band size: 84 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

5% NFDM/TBST dilution buffer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-PSMA antibody [EPR6253] (ab133579)

Primary: ab133579, 1/50 dilution
Dilution buffer: NFDM/TBST, 5%
Sample: LNCaP
Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilution

Anti-PSMA antibody [EPR6253] (ab133579) at 1/50 dilution + LNCaP with NFDM/TBST at 5 %

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Observed band size: 110 kDa why is the actual band size different from the predicted?
Western blot - Anti-PSMA antibody [EPR6253] (ab133579)

Anti-PSMA antibody [EPR6253] (ab133579) at 1/50000 dilution (Unpurified) + LNCaP cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Unpurified ab133579 showing positive staining in Normal prostate tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Unpurified ab133579 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (ab133579)

Unpurified ab133579 showing negative staining in Normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-PSMA antibody [EPR6253] (ab133579)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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