Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6253] to PSMA - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PSMA antibody [EPR6253] - BSA and Azide free
    See all PSMA primary antibodies

  • Description

    Rabbit monoclonal [EPR6253] to PSMA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment aa 1-150 (N terminal).

  • Positive control

    • Human prostate carcinoma tissue; LNCaP cell lysate

  • General notes

    Ab226141 is the carrier-free version of ab133579. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunoprecipitation - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Primary: ab133579, 1/50 dilution
    Dilution buffer: NFDM/TBST, 5%
    Sample: LNCaP
    Secondary: HRP goat anti-rabbit (H+L), 1/1000 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Unpurified ab133579 showing positive staining in Prostatic carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Unpurified ab133579 showing negative staining in Thyroid gland carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Unpurified ab133579 showing positive staining in Normal prostate tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Unpurified ab133579 showing negative staining in Normal colon tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Unpurified ab133579 showing negative staining in Breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    Unpurified ab133579 showing negative staining in Normal spleen tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133579).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

    This IHC data was generated using the same anti-PSMA antibody clone, EPR6253, in a different buffer formulation (cat# ab133579).

    Immunohistochemical staining of paraffin embedded human prostate cancer with purified ab133579 at a working dilution of 1 in 300. HRP polymer for rabbit IgG was used as the secondary antibody, and the sample was counter stained with hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)
    Anti-PSMA antibody [EPR6253] - BSA and Azide free (ab226141)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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