Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)
![Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)](https://www.abcam.com/ps/products/240/ab240975/Images/ab240975-1-ab197587240085ab197587IP.jpg "Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16730] to DOHH - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-DOHH antibody [EPR16730] - BSA and Azide free
See all DOHH primary antibodies -
Description
Rabbit monoclonal [EPR16730] to DOHH - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: LNCaP, U-87 MG and SH-SY5Y whole cell lysates. IP: SH-SY5Y whole cell extract.
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General notes
Ab240975 is the carrier-free version of ab197587. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240975 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16730 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)
DOHH was immunoprecipitated from SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell extract with ab197587 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197587 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: SH-SY5Y whole cell extract 10 µg (Input). Lane 2: ab197587 IP in SH-SY5Y whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197587 in SH-SY5Y whole cell extract
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197587) -
![Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)](https://www.abcam.com/ps/products/240/ab240975/Images/ab240975-2-benefits-of-recombinant-antibodies.png)
Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)
