Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16730] to DOHH - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DOHH antibody [EPR16730] - BSA and Azide free
    See all DOHH primary antibodies

  • Description

    Rabbit monoclonal [EPR16730] to DOHH - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LNCaP, U-87 MG and SH-SY5Y whole cell lysates. IP: SH-SY5Y whole cell extract.

  • General notes

    Ab240975 is the carrier-free version of ab197587. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240975 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)
    Immunoprecipitation - Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)

    DOHH was immunoprecipitated from SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell extract with ab197587 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197587 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: SH-SY5Y whole cell extract 10 µg (Input). Lane 2: ab197587 IP in SH-SY5Y whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197587 in SH-SY5Y whole cell extract

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197587)
  • Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)
    Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DOHH antibody [EPR16730] - BSA and Azide free (ab240975)

© 2021 Astana Biomed Group LLP. All rights reserved.