Anti-DOHH antibody (ab254866)
Key features and details
- Rabbit polyclonal to DOHH
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOHH antibody
See all DOHH primary antibodies -
Description
Rabbit polyclonal to DOHH -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human DOHH aa 1-150.
Database link: Q9BU89 -
Positive control
- IHC-P: Human kidney, duodenum, cerebellum, hippocampus and cerebral cortex tissue. ICC/IF: A-431 cells. WB: DOHH overexpressed HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DOHH antibody (ab254866)PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling DOHH using ab254866 at 4µg/ml (green) in ICC/IF.
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Western blot - Anti-DOHH antibody (ab254866)All lanes : Anti-DOHH antibody (ab254866) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : DOHH overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 69 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (ab254866)Formalin-fixed, paraffin-embedded human kidney tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (ab254866)Formalin-fixed, paraffin-embedded human duodenum tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (ab254866)Formalin-fixed, paraffin-embedded human cerebellum tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (ab254866)Formalin-fixed, paraffin-embedded human hippocampus tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOHH antibody (ab254866)Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for DOHH with ab254866 at a 1/200 dilution in immunohistochemical analysis.
