Anti-ARF1 antibody (ab183576)
Key features and details
- Rabbit polyclonal to ARF1
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
Overview
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Product name
Anti-ARF1 antibody
See all ARF1 primary antibodies -
Description
Rabbit polyclonal to ARF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide corresponding to Human ARF1 aa 150 to the C-terminus (C terminal).
Database link: P84077 -
Positive control
- HeLa, U2 OS, 3T3, NRK and MDCK cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARF1 antibody (ab183576)All lanes : Anti-ARF1 antibody (ab183576) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U2 OS cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : NRK cell lysate
Lane 5 : MDCK cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/1500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
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Immunoprecipitation - Anti-ARF1 antibody (ab183576)Detection of ARF1 in Immunoprecipitates of HeLa cell lysate. Antigen-antibody complexes were formed by incubating 500μg of HeLa whole cell lysate with 2μg ab183576 (lane 2) compared with HeLa cell lysate as a positive control (lane 1). For detection, ab183576 was used at 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-ARF1 antibody (ab183576)Immunofluorescent analysis of HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling ARF1 with ab183576 at 1/100 dilution followed with DyLight 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.
