Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)
![Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)](https://www.abcam.com/ps/products/125/ab125247/Images/ab125247-139416-anti-gapdh-antibody-ga1r-loading-control-western-blot.jpg "Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)")
Key features and details
- Mouse monoclonal [GA1R] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Spodoptera frugiperda (SF9 cells)
- Isotype: IgG1
Overview
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Product name
Anti-GAPDH antibody [GA1R] - Loading Control
See all GAPDH primary antibodies -
Description
Mouse monoclonal [GA1R] to GAPDH - Loading Control -
Host species
Mouse -
Specificity
ab125247 recognizes native and denatured forms of GAPDH. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Rabbit GAPDH.
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Positive control
- Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
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General notes
Store at 4°C (add 0.05% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity chromatography from TCS -
Clonality
Monoclonal -
Clone number
GA1R -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : Human tissue lysate
Lane 2 : Mouse tissue lysate
Lane 3 : Rat tissue lysate
Lane 4 : Rabbit tissue lysate
Lane 5 : Chicken tissue lysate
Lane 6 : Hamster tissue lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
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![Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)](https://www.abcam.com/ps/products/125/ab125247/Images/ab125247-139415-anti-gapdh-antibody-ga1r-loading-control-western-blot.jpg)
Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (ab125247)All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : BL21 bacterial lysate
Lane 2 : Sf9 insect lysate
Lane 3 : Saccharomyces cerevisiae lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
