Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HIP2/LIG antibody [EP1145Y] (ab52930)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1145Y] to HIP2/LIG
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-HIP2/LIG antibody [EP1145Y]
See all HIP2/LIG primary antibodies
Description

Rabbit monoclonal [EP1145Y] to HIP2/LIG
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human HIP2/LIG aa 150-250 (internal sequence). The exact sequence is proprietary.
Positive control
- WB: HCT116, HeLa, Jurkat and Daudi cell lysates. IHC-P: Human liver tissue.
General notes
This product was previously labelled as HIP2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1145Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP2/LIG antibody [EP1145Y] (ab52930)

Ab52930 (1:250) staining human HIP2/LIG in human liver tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-HIP2/LIG antibody [EP1145Y] (ab52930)

All lanes : Anti-HIP2/LIG antibody [EP1145Y] (ab52930) at 1/1000 dilution

Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : UBE2K knockout HCT116 cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 22 kDa
Observed band size: 25 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab52930 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.

ab52930 Anti-HIP2/LIG antibody [EP1145Y] was shown to specifically react with HIP2/LIG in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266899 (knockout cell lysate ab257779) was used. Wild-type and HIP2/LIG knockout samples were subjected to SDS-PAGE. ab52930 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-HIP2/LIG antibody [EP1145Y] (ab52930)

All lanes : Anti-HIP2/LIG antibody [EP1145Y] (ab52930) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : UBE2K knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab52930 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.

ab52930 Anti-HIP2/LIG antibody [EP1145Y] was shown to specifically react with HIP2/LIG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266031 (knockout cell lysate ab257778) was used. Wild-type and HIP2/LIG knockout samples were subjected to SDS-PAGE. ab52930 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-HIP2/LIG antibody [EP1145Y] (ab52930)

Anti-HIP2/LIG antibody [EP1145Y] (ab52930) at 1/100000 dilution + Daudi cell lysate at 10 µg

Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Anti-HIP2/LIG antibody [EP1145Y] (ab52930)

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