Anti-AP2M1 antibody [EP2695Y] (ab75995)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2695Y] to AP2M1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-AP2M1 antibody [EP2695Y]
See all AP2M1 primary antibodies -
Description
Rabbit monoclonal [EP2695Y] to AP2M1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman -
Immunogen
Synthetic peptide within Human AP2M1 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q96CW1 -
Positive control
- WB: MCF-7 and SKBR-3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2695Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/10000 dilution (purified)
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/10000 dilution (purified)
Lane 1 : HEK293 cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-AP2M1 antibody [EP2695Y] (ab75995) at 1/1000 dilution (unpurified)
Lane 1 : MCF-7 cell lysate
Lane 2 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
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