Anti-NUR77 antibody [EPR3209] (ab109180)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3209] to NUR77
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NUR77 antibody [EPR3209]
See all NUR77 primary antibodies -
Description
Rabbit monoclonal [EPR3209] to NUR77 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY5Y cell lysate and HepG2 cells and lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.11% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3209 -
Isotype
IgG -
Research areas
Images
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Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
All lanes : Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
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Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Mouse pituitary at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Rat brain at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -