Anti-NUR77 antibody (ab86130)
Key features and details
- Rabbit polyclonal to NUR77
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUR77 antibody
See all NUR77 primary antibodies -
Description
Rabbit polyclonal to NUR77 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human NUR77 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab96281) -
Positive control
- Recombinant Human NUR77 protein (ab114314) can be used as a positive control in WB. This antibody gave a positive signal in Human liver tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatDogOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).Target
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Function
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. -
Tissue specificity
Fetal muscle and adult liver, brain and thyroid. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 403897 Dog
- Entrez Gene: 3164 Human
- Entrez Gene: 15370 Mouse
- Entrez Gene: 79240 Rat
- Omim: 139139 Human
- SwissProt: P51666 Dog
- SwissProt: P22736 Human
- SwissProt: P12813 Mouse
see all -
Alternative names
- Early response protein NAK1 antibody
- GFRP 1 antibody
- GFRP antibody
see all
Images
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Western blot - Anti-NUR77 antibody (ab86130)Anti-NUR77 antibody (ab86130) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Datasheets and documents
References (1)
ab86130 has been referenced in 1 publication.
- Rehman SU et al. Identification of two novel isoforms of mouse NUR77 lacking N-terminal domains. IUBMB Life 69:106-114 (2017). PubMed: 28111880
Images
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Western blot - Anti-NUR77 antibody (ab86130)Anti-NUR77 antibody (ab86130) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
