Oxalate Assay Kit (Colorimetric) (ab196990)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 20 min
- Sample type: Plasma, Serum, Tissue, Urine
- Sensitivity: 20 µM
Overview
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Product name
Oxalate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Tissue -
Assay type
Quantitative -
Sensitivity
Assay time
2h 20mSpecies reactivity
Reacts with: Mammals, Other speciesProduct overview
Oxalate Assay kit (Colorimetric) (ab196990) is an easy-to-use, sensitive and high throughput adaptable kit. In this assay, Oxalate reacts with oxalate converter and oxalate enzyme mix to form an intermediate that reacts in turn with a highly specific probe to generate color that can be detected at OD=450 nm.
This product can detect Oxalate levels lower than 20 μM.
Oxalate assay protocol summary:
- add samples and standards to wells
- add oxalate convertor and incubate for 1 hr
- add reaction mix and incubate for 1 hr
- analyze with microplate readerNotes
Oxalate (C2O42-), in the form of Oxalic acid is present in many foods and beverages (e.g. spinach, tea etc.). It accumulates in many plant tissues and play role in regulating pH, osmosis and calcium storage. In animals, oxalate is either absorbed from dietary intake or produced from glycolate metabolism in liver. Under normal conditions, the daily oxalate load can be excreted by kidney. However, hereditary defects can cause an increased level of oxalate, which leads to hyperoxaluria and results in the formation of kidney stones. Therefore, measurement of oxalate level is useful for the prevention, diagnosis and monitoring of kidney stones.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Oxalate Assay Buffer 1 x 25ml Oxalate Converter 1 x 0.2ml Oxalate Development Buffer 1 x 15ml Oxalate Enzyme Mix 1 vial Oxalate Probe 1 vial Oxalate Standard 1 vial
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Oxalate Assay Buffer 1 x 25ml Oxalate Converter 1 x 0.2ml Oxalate Development Buffer 1 x 15ml Oxalate Enzyme Mix 1 vial Oxalate Probe 1 vial Oxalate Standard 1 vial
Images
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Functional Studies - Oxalate Assay Kit (Colorimetric) (ab196990) Li, Yuwen et al., PloS one?vol. 12,4 e0176232., Fig 2, doi:10.1371/journal.pone.01762328 weeks old mice were placed for 10 weeks on special egg-white based diets: HPC diet (High phosphate and calcium diet; 20% lactate, 2% calcium, 1.25% phosphate); HP diet (High phosphate diet; 0.6% calcium, 1.20% phosphate); CO diet (Control diet; 0.6% calcium, 0.3% phosphate); WT: wild type; Npt2a: Npt2a-/- mice. Oxylate was measured using 196990
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Example dataOxalate concentration in normal human urine (3 donors, 10 μL sample), normal human serum (10 μL), Kiwi lysate (10 μL) and Spinach lysate (10 μL).
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Example dataOxalate Standard Curve.
