Anti-14-3-3 theta antibody (ab183075)
Key features and details
- Rabbit polyclonal to 14-3-3 theta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 theta antibody -
Description
Rabbit polyclonal to 14-3-3 theta -
Host species
Rabbit -
Specificity
ab183075 detects endogenous level of total 14-3-3 theta protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide within Human 14-3-3 theta aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Genbank: NP_006817
Database link: P27348 -
Positive control
- Mouse brain, Human lymphoma and Human colon cancer tissue lysates; Human A549 and mouse NIH 3T3 cell lysates; Human tonsil tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 theta antibody (ab183075)All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution
Lane 1 : mouse brain tissue lysate
Lane 2 : Human A549 cell lysate
Lane 3 : Human lymphoma tissue lysate
Lane 4 : mouse NIH 3T3 cell lysate
Lane 5 : Human colon cancer tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (ab183075)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)
