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Anti-Plexin A1 antibody [EPR3449] (ab92346)

Price and availability

308 236 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-Plexin A1 antibody [EPR3449] (ab92346)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3449] to Plexin A1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-Plexin A1 antibody [EPR3449]
    See all Plexin A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3449] to Plexin A1
  • Host species

    Rabbit
  • Specificity

    ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Plexin A1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: KS62, HUVEC and HepG2 whole cell lysate (ab7900); fetal brain tissue lysate Flow Cyt: K562 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    This antibody is not purified. It is provided in cell supernatant and storage buffer.
  • Clonality

    Monoclonal
  • Clone number

    EPR3449
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Images

  • Western blot - Anti-Plexin A1 antibody [EPR3449] (ab92346)
    Western blot - Anti-Plexin A1 antibody [EPR3449] (ab92346)
    All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

    Predicted band size: 211 kDa

  • Flow Cytometry - Anti-Plexin A1 antibody [EPR3449] (ab92346)
    Flow Cytometry - Anti-Plexin A1 antibody [EPR3449] (ab92346)

    Flow cytometry analysis of permeabilized K562 cells labeling Plexin A1 with ab92346 at 1/50 (red) campared to a rabbit IgG (negative control) (green).

  • Anti-Plexin A1 antibody [EPR3449] (ab92346)
    Anti-Plexin A1 antibody [EPR3449] (ab92346)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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