Anti-Plexin A1 antibody [EPR3449] (ab92346)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3449] to Plexin A1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-Plexin A1 antibody [EPR3449]
See all Plexin A1 primary antibodies -
Description
Rabbit monoclonal [EPR3449] to Plexin A1 -
Host species
Rabbit -
Specificity
ab92346 is expected to cross react with Plexin A3 and A4 due to 100% immunogen homology with Plexin A3 and 87.5 % with Plexin A4,however not tested experimentally. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human Plexin A1 aa 100-200. The exact sequence is proprietary.
-
Positive control
- WB: KS62, HUVEC and HepG2 whole cell lysate (ab7900); fetal brain tissue lysate Flow Cyt: K562 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Purification notes
This antibody is not purified. It is provided in cell supernatant and storage buffer. -
Clonality
Monoclonal -
Clone number
EPR3449 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Plexin A1 antibody [EPR3449] (ab92346) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 211 kDa
-
Flow cytometry analysis of permeabilized K562 cells labeling Plexin A1 with ab92346 at 1/50 (red) campared to a rabbit IgG (negative control) (green).
-