Anti-ENO3 antibody (ab232759)
Key features and details
- Rabbit polyclonal to ENO3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-ENO3 antibody
See all ENO3 primary antibodies -
Description
Rabbit polyclonal to ENO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human ENO3 aa 1-400. (Expressed in E.coli).
Database link: P13929 -
Positive control
- WB: Pig skeletal muscle and heart lysate; HeLa and HepG2 whole cell lysate; Recombinant human ENO3 protein. IHC-P: Human brain and stomach cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ENO3 antibody (ab232759)All lanes : Anti-ENO3 antibody (ab232759) at 3 µg/ml
Lane 1 : Pig skeletal muscle lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig heart lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO3 antibody (ab232759)Formalin-fixed paraffin-embedded human brain tissue stained for ENO3 using ab232759 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO3 antibody (ab232759)Formalin-fixed paraffin-embedded human stomach cancer tissue stained for ENO3 using ab232759 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab232759)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ENO3 using ab232859 at 20µg/mL in ICC/IF.
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Western blot - Anti-ENO3 antibody (ab232759)Anti-ENO3 antibody (ab232759) at 3 µg/ml + Recombinant human ENO3 protein
Predicted band size: 47 kDa
