Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HEXB antibody [EPR7978] (ab140649)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7978] to HEXB
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-HEXB antibody [EPR7978]
    See all HEXB primary antibodies

  • Description

    Rabbit monoclonal [EPR7978] to HEXB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HEXB aa 400-500. The exact sequence is proprietary.

  • Positive control

    • WB: Recombinant Human HEXB protein (ab114915) Jurkat, HeLa, HepG2 and Caco-2 cell lysates. IHC-P: Human kidney and lung tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-HEXB antibody [EPR7978] (ab140649)
    Western blot - Anti-HEXB antibody [EPR7978] (ab140649)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: HEXB knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab140649 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab140649 was shown to specifically react with HEXB when HEXB knockout samples were used. Wild-type and HEXB knockout samples were subjected to SDS-PAGE. Ab140649 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This image was generated using the unpurified version of the product. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (ab140649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (ab140649)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling HEXB with Purified ab140649 at 1:500 dilution (4.84 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-HEXB antibody [EPR7978] (ab140649)
    Western blot - Anti-HEXB antibody [EPR7978] (ab140649)
    All lanes : Anti-HEXB antibody [EPR7978] (ab140649) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 29,63 kDa
    why is the actual band size different from the predicted?



    The double bands caused by proteolytic processing are consistent with what has been described in PMID 2139028

  • Western blot - Anti-HEXB antibody [EPR7978] (ab140649)
    Western blot - Anti-HEXB antibody [EPR7978] (ab140649)
    All lanes : Anti-HEXB antibody [EPR7978] (ab140649) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa



    This image was generated using the unpurified version of the product. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (ab140649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (ab140649)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HEXB with ab140649 at a 1/25 dilution.

    This image was generated using the unpurified version of the product. 

  • Anti-HEXB antibody [EPR7978] (ab140649)
    Anti-HEXB antibody [EPR7978] (ab140649)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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