Anti-Nucleoporin p62/NUP62 antibody (ab232816)
Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies -
Description
Rabbit polyclonal to Nucleoporin p62/NUP62 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Nucleoporin p62/NUP62 aa 150-450. (Expressed in E.coli).
Database link: P37198 -
Positive control
- ICC/IF: HepG2 cells. IHC-P; Human testis tissue. WB: Pig liver lysate; HepG2, Raji and A549 cell lysates; Recombinant human Nucleoporin p62/NUP62 protein.
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General notes
This product was previously labelled as Nucleoporin p62
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232816 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody (ab232816)
Formalin-fixed, paraffin-embedded human testis tissue stained for Nucleoporin p62/NUP62 using ab232816 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab232816)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Nucleoporin p62/NUP62 (green) using ab232816 at 20 µg/ml in ICC/IF, followed by FITC staining.
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab232816)Anti-Nucleoporin p62/NUP62 antibody (ab232816) at 2 µg/ml + Recombinant human Nucleoporin p62/NUP62 protein
Predicted band size: 53 kDa
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab232816)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab232816) at 3 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : Pig liver lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 53 kDa
