Anti-Nucleoporin p62/NUP62 antibody (ab96134)
Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleoporin p62/NUP62 antibody
See all Nucleoporin p62/NUP62 primary antibodies -
Description
Rabbit polyclonal to Nucleoporin p62/NUP62 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to Human Nucleoporin p62/NUP62 aa 294-502.
Database link: NP_057637 -
Positive control
- WB: A431, H1299, PC-12, Rat2, Neuro-2a, GL261, C8D30, NIH/3T3, BCL-1, RAW 264.7 and C2C12 whole cell lysate. ICC/IF: A431 cells. IHC-P: Mouse fore brain tissue.
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General notes
This product was previously labelled as Nucleoporin p62
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab96134)ab96134, at a 1/200 dilution, staining Nucleoporin p62/NUP62 in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe. -
Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (Rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleoporin p62/NUP62 antibody (ab96134)Paraffin-embedded mouse fore brain tissue stained for Nucleoporin p62/NUP62 using ab96134 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Nucleoporin p62/NUP62 antibody (ab96134)All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Nucleoporin p62/NUP62 antibody (ab96134)Immunofluorescence analysis of A431 cells, staining Nucleoporin p62/NUP62 with ab96134 at 1/200 dilution, demonstrating punctate nuclear staining.
