Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6211] to BMP4 - Low endotoxin, Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free
    See all BMP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR6211] to BMP4 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, A549, HeLa, A431, and Human heart lysates; Human colon tissue.

  • General notes

    ab216455 is the carrier-free version of ab124715.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling BMP4 with Purified ab124715 at 1:400 dilution (2.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

  • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Immunoprecipitation - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)

    ab124715 (purified) at 1:60 dilution (2µg) immunoprecipitating BMP4 in A549 whole cell lysate.
    Lane 1 (input): A549 (Human lung carcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab124715 & A549 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124715 in A549 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

  • Flow Cytometry - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Flow Cytometry - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BMP4 with purified ab124715 at 1:120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

  • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)

    Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) cells labelling BMP4 with purified ab124715 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)

    Unpurified ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    OI-RD Scanning - Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)

    Produced using unpurified ab124715

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124715).

  • Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)
    Anti-BMP4 antibody [EPR6211] - Low endotoxin, Azide free (ab216455)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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