Anti-BMP4 antibody (ab235114)
Key features and details
- Rabbit polyclonal to BMP4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-BMP4 antibody
See all BMP4 primary antibodies -
Description
Rabbit polyclonal to BMP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Rabbit, Cow, Deer -
Immunogen
Recombinant fragment corresponding to Human BMP4 aa 300 to the C-terminus.
Database link: P12644 -
Positive control
- WB: HeLa and A549 cell lysate; Mouse kidney, liver, brain and skeletal muscle lysate; Rat liver and kidney lysate; Whole Zebrafish lysate. IHC-P: Human prostate cancer, lung, small intestine and appendix tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-BMP4 antibody (ab235114) at 1/500 dilution
Lane 1 : Rat liver lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
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Paraffin-embedded human prostate cancer tissue stained for BMP4 using ab235114 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human appendix tissue stained for BMP4 using ab235114 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
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Paraffin-embedded human small intestine tissue stained for BMP4 using ab235114 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
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All lanes : Anti-BMP4 antibody (ab235114) at 1/500 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse skeletal muscle lysate
Lane 3 : Whole Zebrafish lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
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All lanes : Anti-BMP4 antibody (ab235114) at 1/500 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : Rat liver lysate
Lane 4 : Rat kidney lysate
Lane 5 : Whole Zebrafish lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
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Paraffin-embedded human lung tissue stained for BMP4 using ab235114 at 1/100 dilution in immunohistochemical analysis.