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Signal Transduction Growth Factors/Hormones TGF

Anti-BMP4 antibody (ab235114)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-BMP4 antibody (ab235114)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BMP4
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Zebrafish
  • Isotype: IgG

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Overview

  • Product name

    Anti-BMP4 antibody
    See all BMP4 primary antibodies
  • Description

    Rabbit polyclonal to BMP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Rabbit, Cow, Deer
  • Immunogen

    Recombinant fragment corresponding to Human BMP4 aa 300 to the C-terminus.
    Database link: P12644

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa and A549 cell lysate; Mouse kidney, liver, brain and skeletal muscle lysate; Rat liver and kidney lysate; Whole Zebrafish lysate. IHC-P: Human prostate cancer, lung, small intestine and appendix tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Secreted
    • Cancer
    • Growth factors
    • TGF
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Gut specification
    • Developmental Biology
    • Organogenesis
    • Nervous system development
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Images

  • Western blot - Anti-BMP4 antibody (ab235114)
    Western blot - Anti-BMP4 antibody (ab235114)
    All lanes : Anti-BMP4 antibody (ab235114) at 1/500 dilution

    Lane 1 : Rat liver lysate
    Lane 2 : A549 (human lung carcinoma cell line) cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)

    Paraffin-embedded human prostate cancer tissue stained for BMP4 using ab235114 at 1/100 dilution in immunohistochemical analysis.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)

    Paraffin-embedded human appendix tissue stained for BMP4 using ab235114 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)

    Paraffin-embedded human small intestine tissue stained for BMP4 using ab235114 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

  • Western blot - Anti-BMP4 antibody (ab235114)
    Western blot - Anti-BMP4 antibody (ab235114)
    All lanes : Anti-BMP4 antibody (ab235114) at 1/500 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse skeletal muscle lysate
    Lane 3 : Whole Zebrafish lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Western blot - Anti-BMP4 antibody (ab235114)
    Western blot - Anti-BMP4 antibody (ab235114)
    All lanes : Anti-BMP4 antibody (ab235114) at 1/500 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Rat liver lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : Whole Zebrafish lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab235114)

    Paraffin-embedded human lung tissue stained for BMP4 using ab235114 at 1/100 dilution in immunohistochemical analysis.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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