Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)]
    See all GDF11 + GDF8/Myostatin primary antibodies

  • Description

    Rabbit monoclonal [EPR4567(2)] to GDF11 + GDF8/Myostatin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GDF11 + GDF8/Myostatin aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: O95390

  • Positive control

    • WB: HeLa, HEK293 and C6 cell lysate and human heart tissue lysate. C2C12 cell lysate. IHC-P: Human colon adenocarcinoma and cerebral cortex tissues. ICC/IF: A549 cells.

  • General notes

     

     

     This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution + C2C12 (mouse myoblast) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.



    Blocking and Diluting buffer: 5% NFDM /TBST

  • Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + HEK293 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling BMP11 + GDF8/Myostatin with unpurified ab124721 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Flow Cytometry - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

    ab124721 staining GDF11 + GDF8/Myostatin in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/5000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Western blot - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    All lanes : Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Immunocytochemistry/ Immunofluorescence - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

    Immunocytochemistry/Immunofluorescence analysis of A549 cells labelling BMP11 + GDF8/Myostatin with purified ab124721 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    OI-RD Scanning - Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)
    Anti-GDF11 + GDF8/Myostatin antibody [EPR4567(2)] (ab124721)

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