Recombinant human TGF beta 1 protein (Active) (ab50036)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies, WB
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Product name
Recombinant human TGF beta 1 protein (Active)
See all TGF beta 1 proteins and peptides -
Biological activity
The ED50, as determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml), corresponding to a specific activity of ≥ 2 x 107 units/mg.
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Purity
> 98 % SDS-PAGE.
> 98% by HPLC analyses. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS -
Predicted molecular weight
25 kDa -
Amino acids
279 to 390 -
Additional sequence information
TGF-beta1 is a 25.0 kDa protein with each subunit containing 112 aa, linked by a single disulfide bond. It is the mature protein after cleavage of the signal peptide and latency-associated peptide.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH3.0 to a concentration of 50 µg/ml (e.g. 5 µg/100µl). This solution can then be stored at room temperature or at 4oC for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -20oC for future use.
Images
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Western blot - Recombinant human TGF beta 1 protein (Full length) (ab50036)All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate
Lane 2 : NIH/3T3 starved for 18 hours, then treated with 10 ng/ml transforming growth factor-ß (TGF-ß1, ab50036) and 50 µg/ml Heparin sodium salt for 24 hours, whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilutionBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
The level of CTGF expression can be induced by TGF beta treatment (PMID: 17786299).
CTGF is constitutively expressed in HepG2 cells (PMID:15886528).
