Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SEC61A antibody [EPR14379] (ab183046)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14379] to SEC61A
  • Suitable for: IP, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SEC61A antibody [EPR14379]
    See all SEC61A primary antibodies

  • Description

    Rabbit monoclonal [EPR14379] to SEC61A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Daudi and A431 lysates; Human Infiltrating duct carcinoma of breast tissue; A431 and Daudi cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14379

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SEC61A antibody [EPR14379] (ab183046)
    Western blot - Anti-SEC61A antibody [EPR14379] (ab183046)
    All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-SEC61A antibody [EPR14379] (ab183046)
    Immunoprecipitation - Anti-SEC61A antibody [EPR14379] (ab183046)

    Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary antibody: Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow Cytometry - Anti-SEC61A antibody [EPR14379] (ab183046)
    Flow Cytometry - Anti-SEC61A antibody [EPR14379] (ab183046)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Daudi cells labeling SEC61A with ab183046 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61A antibody [EPR14379] (ab183046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61A antibody [EPR14379] (ab183046)

    Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SEC61A antibody [EPR14379] (ab183046)
    Anti-SEC61A antibody [EPR14379] (ab183046)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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