Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SEC61A antibody [EPR14379] (ab183046)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14379] to SEC61A
Suitable for: IP, IHC-P, WB, Flow Cyt
Reacts with: Human

Product name

Anti-SEC61A antibody [EPR14379]
See all SEC61A primary antibodies
Description

Rabbit monoclonal [EPR14379] to SEC61A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, Daudi and A431 lysates; Human Infiltrating duct carcinoma of breast tissue; A431 and Daudi cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14379
Isotype

IgG
Research areas

Western blot - Anti-SEC61A antibody [EPR14379] (ab183046)

All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Daudi cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa
Observed band size: 49 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-SEC61A antibody [EPR14379] (ab183046)

Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary antibody: Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.
Flow Cytometry - Anti-SEC61A antibody [EPR14379] (ab183046)

Flow cytometric analysis of 2% paraformaldehyde-fixed Daudi cells labeling SEC61A with ab183046 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC61A antibody [EPR14379] (ab183046)

Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-SEC61A antibody [EPR14379] (ab183046)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com