Anti-HIP2/LIG antibody - C-terminal (ab177521)
Key features and details
- Rabbit polyclonal to HIP2/LIG - C-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HIP2/LIG antibody - C-terminal
See all HIP2/LIG primary antibodies -
Description
Rabbit polyclonal to HIP2/LIG - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human HIP2/LIG aa 150-200 (C terminal). The exact sequence is proprietary.
Sequence:HVYAGAPVSSP EYTKKIENLC AMGFDRNAVI VALSSKSWDV ETATELLLSN
Database link: P61086 -
Positive control
- 293T, HeLa, Jurkat, TCMK1 and NIH 3T3 whole cell lysates
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General notes
This product was previously labelled as HIP2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-HIP2/LIG antibody - C-terminal (ab177521)
Lane 1: 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated with ab177521 at 6 μg/mg lysate.
Secondary: Anti-rabbit HRP conjugated antibodyLane 2: 293T whole cell lysate (1 mg for IP; 20% of IP loaded) immunoprecipitated with control IgG at 6 μg/mg lysate.
Secondary: Anti-rabbit HRP conjugated antibody -
Western blot - Anti-HIP2/LIG antibody - C-terminal (ab177521)All lanes : Anti-HIP2/LIG antibody - C-terminal (ab177521) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Anti-rabbit HRP conjugated antibody
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 10 seconds
