Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
Key features and details
- Mouse monoclonal [3E8AD9] to GAPDH - Loading Control
- Suitable for: ICC/IF, WB, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-GAPDH antibody [3E8AD9] - Loading Control
See all GAPDH primary antibodies -
Description
Mouse monoclonal [3E8AD9] to GAPDH - Loading Control -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
Positive control
- WB: HepG2 cells IP: HepG2 cells and Human liver mitichondria Flow Cyt: HL-60 cells ICC/IF: Human HDFn cells
-
General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
-
Purification notes
ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3E8AD9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
-
Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305) at 0.125 µg/ml + HepG2 cell lysate
Predicted band size: 36 kDa
-
Immunocytochemistry analysis using ab110305 at 0.5 µg/ml to stain GAPDH in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized). Target locates primarily in the cytosol.
-
Detection of ab110305 by Western Blot of Immunprecipitate.
Staining of GAPDH in HepG2 cells (lane 1) and Humman Liver Mitochondira (lane 2), immunoprecipitated using ab110305. -
Flow cytometric analysis of GAPDH in HL60 cells (blue), using ab110305 at 1 µg/ml. Isotype control antibody (red).