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Neuroscience Neurology process Neurodegenerative disease Huntington's disease

Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)

Price and availability

274 732 ₸

Availability

Order now and get it on Thursday March 18, 2021

Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [3E8AD9] to GAPDH - Loading Control
  • Suitable for: ICC/IF, WB, Flow Cyt, IP
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-GAPDH antibody [3E8AD9] - Loading Control
    See all GAPDH primary antibodies
  • Description

    Mouse monoclonal [3E8AD9] to GAPDH - Loading Control
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HepG2 cells IP: HepG2 cells and Human liver mitichondria Flow Cyt: HL-60 cells ICC/IF: Human HDFn cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    3E8AD9
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Isotype/Loading Controls
    • Loading Controls
    • GAPDH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Western blot - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305) at 0.125 µg/ml + HepG2 cell lysate

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Immunocytochemistry analysis using ab110305 at 0.5 µg/ml to stain GAPDH in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized). Target locates primarily in the cytosol.
  • Immunoprecipitation - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Immunoprecipitation - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Detection of ab110305 by Western Blot of Immunprecipitate.
    Staining of GAPDH in HepG2 cells (lane 1) and Humman Liver Mitochondira (lane 2), immunoprecipitated using ab110305.
  • Flow Cytometry - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Flow Cytometry - Anti-GAPDH antibody [3E8AD9] - Loading Control (ab110305)
    Flow cytometric analysis of GAPDH in HL60 cells (blue), using ab110305 at 1 µg/ml. Isotype control antibody (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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