Anti-Gemin 2 antibody [2E17] (ab6084)
![Anti-Gemin 2 antibody [2E17] (ab6084)](https://www.abcam.com/ps/products/6/ab6084/Images/ab6084-12015-anti-gemin-2-antibody-2e17-flow-cytometry.jpg "Anti-Gemin 2 antibody [2E17] (ab6084)")
Key features and details
- Mouse monoclonal [2E17] to Gemin 2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Gemin 2 antibody [2E17]
See all Gemin 2 primary antibodies -
Description
Mouse monoclonal [2E17] to Gemin 2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine).
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General notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from supernatant. -
Primary antibody notes
Involved in biogenesis of spliceosomal snRNPs, forms complex with Survival of Motor Neurons (SMN) protein, interacts tightly with spliceosomal proteins and spliceosomal snRNAs U1 and U5. -
Clonality
Monoclonal -
Clone number
2E17 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-Gemin 2 antibody [2E17] (ab6084)Overlay histogram showing HeLa cells stained with ab6084 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6084, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
