Anti-SOX6 antibody (ab30455)
Key features and details
- Rabbit polyclonal to SOX6
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-SOX6 antibody
See all SOX6 primary antibodies -
Description
Rabbit polyclonal to SOX6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse SOX6.
Read Abcam's proprietary immunogen policy (Peptide available as ab30530.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab30455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining. Target
-
Function
Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation. -
Tissue specificity
Expressed in a wide variety of tissues, most abundantly in skeletal muscle. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits the transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 55553 Human
- Entrez Gene: 20679 Mouse
- Entrez Gene: 293165 Rat
- Omim: 607257 Human
- SwissProt: P35712 Human
- SwissProt: P40645 Mouse
- SwissProt: Q4V8G3 Rat
- Unigene: 368226 Human
see all -
Alternative names
- HSSOX 6 antibody
- HSSOX6 antibody
- SOX 6 antibody
see all
Images
-
Immunohistochemistry (PFA fixed) - Anti-SOX6 antibody (ab30455)This image is courtesy of Aixa Morales, Cajal Institute, Madrid
IHC (PFA-fixed) image of SOX6 (ab30455) on sections of P18 mouse dentate gyrus. The mice were perfusion fixed with 4% PFA and then post-fixed in 4% PFA for a few hours. The blocks were prepared in agarose/sucrose and cut using a vibratome (50 µm). The sections were permeabilized with 1% Triton X-100 for 30 mins and blocked with 10% FBS for 2 hours at room temperature, then incubated overnight at 4°C with the primary antibody. The primary antibody was used at a 1/100 dilution and detected with donkey anti-rabbit 488 secondary at 1/1000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody (ab30455)This image is courtesy of Eiman Azim, Deb Schuback and Jeff Macklis, Massachusetts General Hospital - Harvard Medical School Center for Nervous System Repairab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
References (36)
ab30455 has been referenced in 36 publications.
- Lv L et al. SOX6 suppresses the development of lung adenocarcinoma by regulating expression of p53, p21CIPI , cyclin D1 and ß-catenin. FEBS Open Bio 10:135-146 (2020). PubMed: 31729835
- Jaiswal PK et al. Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9. Sci Rep 10:7790 (2020). PubMed: 32385306
- Simmons SC et al. Determination of circuit-specific morphological adaptations in ventral tegmental area dopamine neurons by chronic morphine. Mol Brain 12:10 (2019). PubMed: 30736837
- Takimoto A et al. Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. Sci Rep 9:4605 (2019). PubMed: 30872687
- Wu L et al. Myricetin improves endurance capacity by inducing muscle fiber type conversion via miR-499. Nutr Metab (Lond) 16:27 (2019). PubMed: 31073320
Images
-
Immunohistochemistry (PFA fixed) - Anti-SOX6 antibody (ab30455) This image is courtesy of Aixa Morales, Cajal Institute, Madrid
IHC (PFA-fixed) image of SOX6 (ab30455) on sections of P18 mouse dentate gyrus. The mice were perfusion fixed with 4% PFA and then post-fixed in 4% PFA for a few hours. The blocks were prepared in agarose/sucrose and cut using a vibratome (50 µm). The sections were permeabilized with 1% Triton X-100 for 30 mins and blocked with 10% FBS for 2 hours at room temperature, then incubated overnight at 4°C with the primary antibody. The primary antibody was used at a 1/100 dilution and detected with donkey anti-rabbit 488 secondary at 1/1000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody (ab30455) This image is courtesy of Eiman Azim, Deb Schuback and Jeff Macklis, Massachusetts General Hospital - Harvard Medical School Center for Nervous System Repairab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
