Anti-UGDH antibody (ab246999)
Key features and details
- Rabbit polyclonal to UGDH
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-UGDH antibody
See all UGDH primary antibodies -
Description
Rabbit polyclonal to UGDH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Recombinant fragment corresponding to Human UGDH aa 400-500.
Database link: O60701 -
Positive control
- WB: A549, NIH/3T3 and NBT-II whole cell lysates. IHC-P: Human rectum, liver and prostate tissue. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-UGDH antibody (ab246999)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for UGDH (green) using ab246999 at 4 μg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (ab246999)Formalin-fixed, paraffin-embedded human prostate tissue stained for UGDH with ab246999 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (ab246999)Formalin-fixed, paraffin-embedded human liver tissue stained for UGDH with ab246999 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (ab246999)Formalin-fixed, paraffin-embedded human rectum tissue stained for UGDH with ab246999 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGDH antibody (ab246999)Formalin-fixed, paraffin-embedded human cerebral cortex tissue shows absence of immunoreactivity for UGDH when incubated with ab246999 at a 1/500 dilution (negative control).
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Western blot - Anti-UGDH antibody (ab246999)All lanes : Anti-UGDH antibody (ab246999) at 0.4 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Predicted band size: 55 kDaCorresponding UGDH RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
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Western blot - Anti-UGDH antibody (ab246999)All lanes : Anti-UGDH antibody (ab246999) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 55 kDa