Protein Nitration Assay Kit (Nitrotyrosine) (ab242294)
Key features and details
- Platform: Western blot
- Sample type: Cell Lysate, Plasma, Purified protein, Serum
Overview
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Product name
Protein Nitration Assay Kit (Nitrotyrosine) -
Sample type
Serum, Plasma, Cell Lysate, Purified protein -
Product overview
Nitric oxide (NO) was initially identified as a muscle relaxation factor, mediating its effects on smooth muscle by coordinating the heme moiety of guanylate cyclase, leading to enhanced production of cGMP. Besides smooth muscle relaxation, NO also influences a variety of biological systems including cell proliferation, apoptosis, neurotoxicity, Parkinson disease, extracellular matrix remodeling. Many of its effects are mediated through protein nitration.
Peroxynitrite (ONOO– ) is formed in the chemical reaction between nitric oxide and superoxide. ONOO– nitrates free tyrosine and tyrosine residues in proteins; modifications that are used as markers of ONOO– action under conditions of cellular damage and in numerous diseases.
ab242294 offers a simple and complete system for the detection of nitrotyrosine in proteins. This kit also includes Nitrated BSA as positive control. Each kit provides sufficient quantities to perform at least 10 blots (7.5 cm x 8.5 cm).
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Tested applications
Suitable for: WBmore details -
Platform
Western blot
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 tests Nitrotyrosine Immunoblot Control 1 x 100µl Rabbit Anti-Nitrotyrosine Antibody 1 x 100µl Secondary Antibody, HRP Conjugate 1 x 100µl -
Research areas
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Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
