Recombinant Human p53 protein (ab43615)
Key features and details
- Expression system: Escherichia coli
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
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Product name
Recombinant Human p53 protein
See all p53 proteins and peptides -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDI EQWFTEDPGP DEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVP SQKTYQGSYGFRLGFLHSGTAK SVTCTYSPALNKMFCQLAKTCPVQLW VDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHE RCSDSDGLAPPQHL IRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS SC MGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKK GEPHHELP PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM FRELNEALELKDAQAGKEPG GSRAHSSHLKSKKGQSTSRHKKLMFKTE GPDSD -
Amino acids
1 to 393 -
Tags
GST tag N-Terminus
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Description
Recombinant Human p53 protein
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.00292% EDTA, 0.00385% DTT, 0.00174% PMSF, 0.0038% EGTA
Images
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SDS-PAGE - Recombinant Human p53 protein (ab43615)
Lane 1 : MW marker. Lane 2 : ab43615 100ng. Observed band size : 72 kDa - p53 full length (44 kDa) + GST (28 kDa) = 72kDa
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Western blot - Recombinant Human p53 protein (ab43615)Recombinant Human p53 protein (ab43615)
Observed band size: 79 kDa why is the actual band size different from the predicted?
