Mouse MMP10 ELISA kit (ab277454)
Key features and details
- Sensitivity: 2 pg/well
- Range: 1.37 pg/well - 25 pg/well
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MMP10 ELISA kit
See all MMP10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/well -
Range
1.37 pg/well - 25 pg/well -
Recovery
Sample specific recovery Sample type Average % Range Serum 112.3 93% - 147% Cell culture media 112.9 100% - 124% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MMP10 ELISA Kit (ab277454) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MMP10 in serum (plasma is not recommended for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Mouse MMP10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated Anti-Mouse MMP10 Antibody 2 vials Anti-Mouse MMP10 coated Microplate 1 unit Mouse MMP10 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Matrix metallopeptidase 10 (stromelysin 2)
- Matrix metalloprotease 10
- Matrix metalloproteinase 10
see all -
Database links
- Entrez Gene: 17384 Mouse
- SwissProt: O55123 Mouse
- Unigene: 14126 Mouse
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.