Collagenase Activity Assay Kit (Colorimetric) (ab196999)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 25 min
- Sample type: Cell Lysate, Purified protein
- Sensitivity: 20 µU
Overview
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Product name
Collagenase Activity Assay Kit (Colorimetric)
See all Collagenase kits -
Detection method
Colorimetric -
Sample type
Cell Lysate, Purified protein -
Assay type
Enzyme activity -
Sensitivity
> 20 µU -
Assay time
0h 25m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Bacteria -
Product overview
Collagenase Activity Assay Kit (ab196999) provides a quick and easy way to determine activity of Collagenase. The assay measures collagenase activity using a synthetic peptide (FALGPA) that mimics the structure of collagen. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum type I-XI. This assay can also be used for screening or characterization of collagenase inhibitors.
The limit of detection for this assay is 0.02 mU Collagenase.
Collagenase assay protocol summary:
- add samples and controls to wells
- add reaction mix
- analyze with microplate reader in kinetic mode for 5-15 min -
Notes
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, which is a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren’s contracture, an affliction characterized by a thickening of connective tissue.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Collagenase Red 1 x 1ml Collagenase Assay Buffer 1 x 20ml Collagenase Substrate 1 x 4ml Inhibitor 1 x 50µl -
Research areas
Images
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Functional Studies - Collagenase Activity Assay Kit (Colorimetric) (ab196999)
Collagenase activity over 10 minutes.
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Functional Studies - Collagenase Activity Assay Kit (Colorimetric) (ab196999)Enzyme activity of collagenase and inhibition by 10 mM (1,10)-Phenanthroline. Assay was performed following the kit protocol.
