Collagenase Inhibitor Assay Kit (Fluorometric) (ab211108)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Collagenase Inhibitor Assay Kit (Fluorometric)
See all Collagenase kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Semi-quantitative -
Product overview
Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and reliable test for high-throughput screening of collagenase inhibitors. The assay uses self-quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorescence microplate reader at Ex/Em = 490/520 nm (515 nm cutoff). In the presence of a collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.
This kit can be used to screen/study/characterize potential inhibitors of Collagenase.
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Notes
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren’s contracture, an affliction characterized by a thickening of connective tissue.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Collagenase 1 x 30µl Collagenase Assay Buffer 1 x 25ml Collagenase Substrate (40 µg) 1 vial Inhibitor (1,10)-Phenanthroline (400 mM) 1 x 50µl
Images
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Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108)
Collagenase Inhibitor Screening Kit (ab211108). Typical inhibition curve of Collagenase activity by collagenase inhibitor. IC50 of (1,10)-Phenanthroline was determine to be 110.5 µM. Assay was performed following the kit protocol.
