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Anti-CACNA1A antibody [EPR4458(N)] (ab181371)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4458(N)] to CACNA1A
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CACNA1A antibody [EPR4458(N)]
    See all CACNA1A primary antibodies
  • Description

    Rabbit monoclonal [EPR4458(N)] to CACNA1A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CACNA1A aa 2050-2150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O00555
    (Peptide available as ab205923)

  • Positive control

    • Human fetal brain, Human cerebellum and C6 lysates; Permeabilized C6 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4458(N)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Channels
    • P / Q / R -Type
    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Channels
    • L-type
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Images

  • Western blot - Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    Western blot - Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    All lanes : Anti-CACNA1A antibody [EPR4458(N)] (ab181371) at 1/5000 dilution

    Lane 1 : Human cerebullum lysate
    Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 282 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    The 220 and 170 kDa is full-length a1A subunit species. The 60 kDa is a C-terminal fragment of the a1A subunit.(PMID:16595610).

  • Western blot - Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    Western blot - Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    All lanes : Anti-CACNA1A antibody [EPR4458(N)] (ab181371) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 282 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    The 220 and 170 kDa is full-length a1A subunit species. The 60 kDa is a C-terminal fragment of the a1A subunit.(PMID:16595610).

  • Western blot - Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    Western blot - Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    All lanes : Anti-CACNA1A antibody [EPR4458(N)] (ab181371) at 1/1000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human cerebellum lysates
    Lane 3 : C6 lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 282 kDa

  • Anti-CACNA1A antibody [EPR4458(N)] (ab181371)
    Anti-CACNA1A antibody [EPR4458(N)] (ab181371)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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