Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Wnt7a antibody [EPR23471-125] (ab274321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23471-125] to Wnt7a
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Wnt7a antibody [EPR23471-125]
    See all Wnt7a primary antibodies

  • Description

    Rabbit monoclonal [EPR23471-125] to Wnt7a

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HaCaT and BxPC-3 whole cell lysates; Mouse P7 hippocampus tissue lysate. ICC: HaCaT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23471-125

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Wnt7a antibody [EPR23471-125] (ab274321)
    Western blot - Anti-Wnt7a antibody [EPR23471-125] (ab274321)
    All lanes : Anti-Wnt7a antibody [EPR23471-125] (ab274321) at 1/1000 dilution

    Lane 1 : HaCaT (human skin keratinocyte) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : Mouse P7 hippocampus tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
    Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 42/40 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 25978974, 30143647, 30770219). 

    Negative control: HeLa cells (PMID: 25978974).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

     

  • Immunocytochemistry - Anti-Wnt7a antibody [EPR23471-125] (ab274321)
    Immunocytochemistry - Anti-Wnt7a antibody [EPR23471-125] (ab274321)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HaCaT (human skin keratinocyte) and HeLa (human cervix adenocarcinoma epithelial cell) whole cells labelling Wnt7a with ab274321 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in HaCaT cells. Low expression cell line: HeLa cells (PMID: 25978974). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

  • Anti-Wnt7a antibody [EPR23471-125] (ab274321)
    Anti-Wnt7a antibody [EPR23471-125] (ab274321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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