Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)
![Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)](https://www.abcam.com/ps/products/79/ab79079/Images/ab79079-380892-anti-epcam-antibody-vu-1d9-biotin-ihc-human-breast.jpg "Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)")
Key features and details
- Biotin Mouse monoclonal [VU-1D9] to EpCAM
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-EpCAM antibody [VU-1D9]
See all EpCAM primary antibodies -
Description
Biotin Mouse monoclonal [VU-1D9] to EpCAM -
Host species
Mouse -
Conjugation
Biotin -
Specificity
We have data to indicate that this antibody may not cross react with Rat or ferret. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
ab79079 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Tissue, cells or virus corresponding to Human EpCAM.
Database link: P16422 -
Positive control
- Human colon carcinoma tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 29th September 2017 and product received after this date will be from tissue culture supernatant.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.41% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
VU-1D9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)
ab79079 at 1-2 µg/mL staining EpCAM in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.
