Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] (ab179438)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7862] to Thyroid Hormone Receptor alpha
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Thyroid Hormone Receptor alpha antibody [EPR7862]
See all Thyroid Hormone Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR7862] to Thyroid Hormone Receptor alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Thyroid Hormone Receptor alpha aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P10827 -
Positive control
- WB: Jurkat, MEF, NIH/3T3, Molt-4 and MCF-7 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7862 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] (ab179438) at 0.546 µg/ml
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte)
Lane 2 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate
Lane 3 : MEF (Mouse embryonic fibroblast)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 55 kDa
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All lanes : Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] (ab179438) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
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