Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-39-2C3] to Baf180 - BSA free
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-Baf180 antibody [BL-39-2C3] - BSA free
See all Baf180 primary antibodies
Description

Rabbit monoclonal [BL-39-2C3] to Baf180 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Baf180 aa 1-293.
Database link: Q86U86
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272073 is the carrier-free version of ab243876. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-39-2C3
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)

Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Baf180 using ab243876 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)

Formalin-fixed, paraffin-embedded human Breast tissue stained for Baf180 using ab243876 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
Immunoprecipitation - Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)

Baf180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution.

Lane 1: ab243876 IP in HEK-293T whole cell lysate.

Lane 2: Contol IgG in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).
Anti-Baf180 antibody [BL-39-2C3] - BSA free (ab272073)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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